Skip to content

Development of transgenic teak (Tectona grandis) expressing a cry1AB genefor control of the skeletoniser

បោះពុម្ពផ្សាយដោយ៖ Voices for Mekong Forest

Teak (Tectona grandis) is one of the exotic forest species that has been planted in Malaysia. Teak skeletoniser, Paliga damastesalis, causes minor to considerable damage to teak trees in plantations. We have utilised and modifed a cloned cry1Ab gene to construct a chimeric gene capable of expression in plant cells. Using the Biolistic® particle delivery system, we then transferred the cry1Ab gene into teak cells and regenerated transgenic plants. Te presence and integration of the introduced genes was confrmed by growth of tissue on selection media (MS-10Hm), and by molecular analyses, i.e. Polymerase Chain Reaction (PCR) and PCR-Southern Blotting. PCR analyses for the cry1A(b) gene was carried out on 90 putative transformed teak plants. Te expected internal fragment of 746bp for cry1Ab gene was successfully amplifed from some of the transformants. Te PCR-Southern analyses confrmed the presence of this gene, and RT-PCR analyses confrmed the expression of this transgene in the positive transformants. A bioassay result showed that about 57 cm2 - 66 cm2 of the non-transgenic leaves area showed skeletonisa-tion, whereas only 3 cm2 - 6 cm2 of the transgenic leaves were skeletonised. Te size of the larvae feeding on the control teak leaves increased about 13 to 18 mm. In contrast, larvae feeding on transgenic teak leaves were signifcantly smaller, only 5 to 8 mm. Currently the transgenic teak trees have been successfully planted in a greenhouse at the Forest Research Institute of Malaysia (FRIM)'s nursery.

ធនធានទិន្នន័យ (1)

មើល​ធនធានទិន្នន័យ - Development of transgenic teak (Tectona grandis) expressing a cry1AB genefor control of the skeletoniser

Additional Info

Field Value
ប្រភេទឯកសារ Reports, journal articles, and research papers (including theses and dissertations)
ភាសារបស់ឯកសារ
  • ភាសាអង់គ្លេស
ប្រធានបទ
  • Forest policy and administration
  • Logging and timber
តំបន់ភូមិសាស្រ្ត (ការលាតសន្ធឹងក្នុងលំហ)
  • ឥណ្ឌា
  • ឥណ្ឌូនេស៊ី
  • ឡាវ
  • មីយ៉ាន់ម៉ា
  • ថៃ
សិទ្ធិ Yes
កំហិតនៃការចូល និងប្រើប្រាស់

Subject to copyright constraints by ResearchGate

កំណែ/បោះពុម្ពលើកទី 2011
អាជ្ញាប័ណ្ណ unspecified
ទំនាក់ទំនង

Norlia Basherudin Institut Penyelidikan Perhutanan Malaysia (FRIM), 52109 Kepong, Selangor Darul Ehsan, Malaysia. G : 3.2464539,101.643837 T : 603-62797000 F : 603 62731314 E : feedback@frim.gov.my

អ្នកនិពន្ធ (ជាបុគ្គល) A. Norwati
សហអ្នកនិពន្ធ (បុគ្គល) Norlia Basherudin, H. Mohd Rosli, Norwati Muhammad, R. Abdullah
កន្លែងបោះពុម្ភផ្សាយ Malaysia
អ្នកបោះពុម្ពផ្សាយ Asia-Pacific Journal of Molecular Biology and Biotechnology
កាលបរិច្ឆេទបោះពុម្ព 2011
ការបង់លេខ 149
ពាក្យគន្លឹះ V4MF,FLEGT
កាលបរិច្ឆេទផ្ទុកឡើង មេសា 16, 2018, 18:17 (UTC)
កាលបរិច្ឆេទកែប្រែ មេសា 19, 2018, 04:43 (UTC)